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<p class="MsoNormal">Dear EEGLAB community,<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">I am currently working on a dataset from which I need to get Beta band coherence (motor cortex to muscle coupling). My processing pipeline for the dataset so far has been:<o:p></o:p></p>
<p class="MsoListParagraph" style="text-indent:-.25in;mso-list:l0 level1 lfo1"><![if !supportLists]><span style="mso-list:Ignore">1-<span style="font:7.0pt "Times New Roman"">
</span></span><![endif]>Import data<o:p></o:p></p>
<p class="MsoListParagraph" style="text-indent:-.25in;mso-list:l0 level1 lfo1"><![if !supportLists]><span style="mso-list:Ignore">2-<span style="font:7.0pt "Times New Roman"">
</span></span><![endif]>Add channels, Optimize Center, Set channel types<o:p></o:p></p>
<p class="MsoListParagraph" style="text-indent:-.25in;mso-list:l0 level1 lfo1"><![if !supportLists]><span style="mso-list:Ignore">3-<span style="font:7.0pt "Times New Roman"">
</span></span><![endif]>Cleanline (default settings, to EEG and EMG)<o:p></o:p></p>
<p class="MsoListParagraph" style="text-indent:-.25in;mso-list:l0 level1 lfo1"><![if !supportLists]><span style="mso-list:Ignore">4-<span style="font:7.0pt "Times New Roman"">
</span></span><![endif]>FIR 1-250Hz (pop_eegfiltnew, to EEG and EMG)<o:p></o:p></p>
<p class="MsoListParagraph" style="text-indent:-.25in;mso-list:l0 level1 lfo1"><![if !supportLists]><span style="mso-list:Ignore">5-<span style="font:7.0pt "Times New Roman"">
</span></span><![endif]>Segment data in epochs (0.5 seconds of epochs, for coherence purposes I noticed in most studies the general number of windows is usually above 170, thus to achieve the same statistical relevance I needed to have windows of 0.5s rather
than 1second. I am a little confused about which window length should be preferred for beta band coherence)<o:p></o:p></p>
<p class="MsoListParagraph" style="text-indent:-.25in;mso-list:l0 level1 lfo1"><![if !supportLists]><span style="mso-list:Ignore">6-<span style="font:7.0pt "Times New Roman"">
</span></span><![endif]>Remove noisy channel (if more than 10% of data is “bad”, then I remove channel)<br>
6a)pop_eegthresh on EEG channels, with limits of -50 to 50 uV<br>
6b)pop_jointprob on EEG channels, single-channel std:6, All-channel std:2<br>
if a channel is generally responsible for about 10% of rejected epochs, I reject channel<o:p></o:p></p>
<p class="MsoListParagraph" style="text-indent:-.25in;mso-list:l0 level1 lfo1"><![if !supportLists]><span style="mso-list:Ignore">7-<span style="font:7.0pt "Times New Roman"">
</span></span><![endif]>Interpolate rejected channels, pop_interp ;Spherical method<o:p></o:p></p>
<p class="MsoListParagraph" style="text-indent:-.25in;mso-list:l0 level1 lfo1"><![if !supportLists]><span style="mso-list:Ignore">8-<span style="font:7.0pt "Times New Roman"">
</span></span><![endif]>Average reference EEG channels(for ICA and coherence calculations, our actual reference is between FCZ and CZ)<o:p></o:p></p>
<p class="MsoListParagraph" style="text-indent:-.25in;mso-list:l0 level1 lfo1"><![if !supportLists]><span style="mso-list:Ignore">9-<span style="font:7.0pt "Times New Roman"">
</span></span><![endif]>Run ICA on EEG channels only ( I have read that EOG and ECG channels can be included, but they are bipolar readings with a different reference from EEG, should I include them regardless?<o:p></o:p></p>
<p class="MsoListParagraph" style="text-indent:-.25in;mso-list:l0 level1 lfo1"><![if !supportLists]><span style="mso-list:Ignore">10-<span style="font:7.0pt "Times New Roman"">
</span></span><![endif]>Use MARA toolbox to get a general sense of how I should feel about some IC’s , also because I prefer the MARA spectrum plot over the default GUI<o:p></o:p></p>
<p class="MsoListParagraph" style="text-indent:-.25in;mso-list:l0 level1 lfo1"><![if !supportLists]><span style="mso-list:Ignore">11-<span style="font:7.0pt "Times New Roman"">
</span></span><![endif]>Reject IC components related to EMG artifacts (facial movements, neck, jaw, eyes), from 64 components I reject ~20<o:p></o:p></p>
<p class="MsoListParagraph" style="text-indent:-.25in;mso-list:l0 level1 lfo1"><![if !supportLists]><span style="mso-list:Ignore">12-<span style="font:7.0pt "Times New Roman"">
</span></span><![endif]>Reject epochs based on the Components (jointprob 6,2)<o:p></o:p></p>
<p class="MsoListParagraph" style="text-indent:-.25in;mso-list:l0 level1 lfo1"><![if !supportLists]><span style="mso-list:Ignore">13-<span style="font:7.0pt "Times New Roman"">
</span></span><![endif]>Run ICA again, reject EMG components again<o:p></o:p></p>
<p class="MsoListParagraph" style="text-indent:-.25in;mso-list:l0 level1 lfo1"><![if !supportLists]><span style="mso-list:Ignore">14-<span style="font:7.0pt "Times New Roman"">
</span></span><![endif]>Most of the time by this point I have retained ~75-85% of dataset, and I use this dataset to do coherence analysis. I have read that some have suggested instead to export the ICA matrix of the processed data, and apply it to the raw
data?<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Am I rejecting too many IC’s from the data, or would this data produce reliable results?<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Thanks in advance,<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Armand Hoxha<o:p></o:p></p>
<p class="MsoNormal">Biomedical Engineer, HPEL<o:p></o:p></p>
<p class="MsoNormal">Kessler Foundation<o:p></o:p></p>
<p class="MsoNormal">1199 Pleasant Valley Way, West Orange, NJ 07052<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
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